Binding of annexin V by T cells from patients with B-NHL. (A) Freshly isolated lymphocytes from PBL of a healthy donor were stained for binding of annexin V before (i), or, to promote apoptosis, after culture for 48 hours on immobilized anti-CD3 mAb at 10 (ii) and 100 (iii) ng/mL. (B) PBL, or involved LNs, or BM of 5 patients with FL or CLL were stained for binding of annexin V. Lymphocytes were gated on CD3⁺ T cells and then analyzed for CD4 or CD8 expression versus annexin V binding. Numbers in each dot plot refer to the percentage of positive cells in each quadrant. PT indicates patient.