Figure 2.
![Figure 2. Genetic inactivation of p110δ impairs in vitro B-cell proliferation and induction of cell-cycle regulators. (A) Purified splenic B cells were cultured with or without anti-IgM for 48 hours, followed by measurement of [3H]-thymidine incorporation. Error bars indicate SD. (B-C) Cell lysates were prepared from WT and p110δD910A/D910A B cells at the indicated time points following anti-IgM stimulation, followed by assessment of expression of cell-cycle regulators by immunoblotting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/2/10.1182_blood-2005-07-3041/4/zh80020689410002.jpeg?Expires=1724239093&Signature=K4JGYAtfbAl4mbVzjjgiZM4Frr18gwXGMoMfWfXLW6uMuaFNrOKJSDLIfJuD~erBOEjPU0omocoMUXSAIhGqT6kCGXNQqRE4caJLaHvhaMFL--PfUXUZ4VUPIwzOLspm1UOt9USllP3aYUBtD8KP~1W05w-IRmiBQWUanjm-Qkc8c0sfs0K3DSNPEhB2iMS1TuB11pWkXu73iHx~qrccw3vPHXFreSsdozUOPqQfpgMoqk47jbq61YufYPzXCbzNQMcZDdet-39ELERiTpk0m0YaYcDZLPhNazwlglOi2hcMjGE-w-8IYVDCQkoL~6TH9hpV68VNBtB7CkYUFYplGg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genetic inactivation of p110δ impairs in vitro B-cell proliferation and induction of cell-cycle regulators. (A) Purified splenic B cells were cultured with or without anti-IgM for 48 hours, followed by measurement of [3H]-thymidine incorporation. Error bars indicate SD. (B-C) Cell lysates were prepared from WT and p110δD910A/D910A B cells at the indicated time points following anti-IgM stimulation, followed by assessment of expression of cell-cycle regulators by immunoblotting.
