Eyelid opening, macrophage morphology, and B-cell distribution in Csf1^op/Csf1^op; TgSGP/+ and Csf1^op/Csf1^op; TgSPP/+ mice. (A) Incomplete eyelid opening in Csf1^op/Csf1^op and Csf1^op/Csf1^op; TgSGP/+ mice. H&E staining of eyelids from 6-week-old mice. Top panels: narrow gap between the eyelids marked by an asterisk in Csf1^op/Csf1^op and Csf1^op/Csf1^op; TgSGP4/+ mice. Middle panels: chronic active blepharitis with hyperkeratosis marked by arrowhead. Bottom panels: meibomian gland (MG) hyperplasia of the eyelids of these same mice compared with the eyelids of wt mice. (B) Lack of apoptosis in the eyelids of 2-day-old Csf1^op/Csf1^op mice compared with wt control mice. Brown dots indicate TUNEL-positive cells. (C) Reduced dendritic morphology of F4/80-stained macrophages in 3-month-old Csf1^op/Csf1^op; TgSGP/+ liver. (D) B220⁺ cells as a percentage of total white blood cells in blood, spleen, and bone marrow of mice expressing TgSPP and TgSGP on Csf1^op/Csf1^op background. Data are given as mean ± SD. ^*P < .05 and †P < .08, for comparison with wt.