The effect of sTF-annV on the tail-bleeding time. (A) Mice (n = 13) were given a subcutaneous injection of enoxaparin sodium (20 mg/kg), and 2 hours later they were anesthetized and the tail-bleeding time was determined as described in “Materials and methods.” Immediately upon the cessation of bleeding, the animals were injected intravenously with sTF-annV (90 μg/kg) in TBS. Five minutes later, the bleeding time was measured again. The differences were significant at a level of P = .01 (paired 2-tailed t test). (B) Tail-bleeding times were measured as described in panel A. Two hours after receiving subcutaneous enoxaparin (or saline), animals were injected intravenously with sTF-annV (90 μg/kg) or equivalent molar amounts of sTF, annexin V, or both sTF and annexin V. Tail-bleeding times were measured 10 minutes later. sTF-annV shortened the bleeding time of mice that did not receive enoxaparin (P > .2) as well as those that did (P < .001, 2-tailed unpaired t test with Welch correction). Two animals were studied in each of the groups that did not receive enoxaparin, and 4 in each of the enoxaparin-treated groups. Shown are the means ± SEM.