Figure 4.
Autoactivation of factor VIIa in the presence of native TF, sTF-annV, or sTF. Factor VII (80 nM) was added to equimolar concentrations of native TF (▪), sTF (▾), or sTF-annV (•) in the presence of PC/PS (810 μM, PC/PS molar ratio = 4:1) and CaCl2. At the indicated times, aliquots were removed and the reaction was stopped by dilution in EDTA-containing solutions. The factor VIIa content of each sample was then determined by measuring the rate of hydrolysis of Chromozym t-PA in the presence of added sTF and CaCl2. Shown are means ± SEM.
