Phospholipid binding of sTF-annV and sTFAA-annV. The ability of sTF-annV (▾) and sTFAA-annV (▴) to bind PC/PS vesicles was compared with that of annexin V (▪) by assessing the ability of each protein to displace FITC-annexin V from a phospholipid suspension, as described in “Materials and methods.” The curves were computer-generated using nonlinear regression and a one-site ligand-binding equation. Data are expressed as the percent (mean ± SEM) of EDTA-elutable fluorescence.