Influence of recombinant proteins on plasma coagulation. (A) The coagulation time of citrated human plasma with different concentrations of native TF (▪), sTF (▾), or sTF-annV (•). The total amount of PC/PS added to each sample was 10 μM (PC/PS molar ratio, 4:1). Shown are means ± SEM. (B) Plasma coagulation times with sTF (▾), annexin V (▴), both sTF and annexin V (♦), or sTF-annV (•) measured using a dilute partial thromboplastin time protocol, as described in “Materials and methods.” (C) Plasma coagulation times, in the absence of added phospholipids, with different concentrations of sTF (▾), annexin V (▴), sTF-annV (•), or sTFAA-annV (○). Shown are means ± SEM.