Flow cytometric dot-plot analyses. Analyses of TLR2 (left column), TLR4 (middle column), and TLR9 (right column) expression on human platelets. Platelets were stained with the indicated PE-labeled antibody and were acquired through a live platelet gate based on forward and side scatter. Data are presented as dot plots of forward scatter and TLR (FL2) fluorescence. The horizontal marker was set based on background fluorescence using fluorescent isotype controls. The numbers in the top and bottom right quadrants refer to the percentage of cells in each quadrant. A typical example is shown.