Figure 1.
Cell lines derived from Tal1 tumors are sensitive to a γ-secretase inhibitor.Tal1 tumor cell lines were treated with 1 μM DAPT or DMSO (control) for 6 days, and the DNA content of propidium iodide-stained cell populations was determined by flow cytometry. (A-D) Numbers over the cell populations indicate the percentages of cells in sub-G0/G1, G0/G1, S, and G2/M phases. Tal1 tumor cells were untreated or treated with DAPT for 40 hours. (E) Western blot analysis using an antibody that specifically recognizes the activated form of notch1. A Tal1 T-ALL cell line was untreated or treated with DAPT for 24 or 48 hours. (F) RT-PCR analysis was performed with primers specific for Deltex, Hes1, and GAPDH.
