Immature phenotype of Tg NK cells. (A) BM and spleen cells from WT and Tg mice were stained for Mac-1, NK1.1, and CD3. Shown are profiles of Mac-1 expression on gated CD3^- cells, and the numbers represent the percentages of cells in each quadrant. (B) BM and spleen cells were stained for CD43, DX5, NK1.1, and CD3. Gated NK1.1⁺ CD3^- cells are shown, and the numbers represent the percentages of CD43^hi cells. (C) BM and spleen cells were stained for integrin αv, DX5, NK1.1, and CD3. Gated NK1.1⁺ CD3^- cells are shown, and the numbers represent the percentages of cells in each quadrant. More than 5 mice were analyzed for each marker.