Doxycycline induction of KSHV vGPCR in BC3.14 cells results in increases in vGPCR message similar to levels obtained with lysis-inducing chemicals. BC3.14 cells were treated for 48 hours with TPA (20 ng/mL), butyrate (1 mM and 2 mM), or 2 μg/mL doxycycline (48 and 96 hours). Quantitative RT-PCR was then done using primers for KSHV vGPCR. Results were normalized to untreated cells in lane 1. Error bars indicate SD.