KSHV vGPCR-induced cell-cycle arrest is not mediated by p53. (A) BC3.14 cells were exposed to doxycycline for the specified length of time to induce vGPCR, after which protein lysates (30 μg) were loaded onto 12% SDS-PAGE gels and probed for p53 and mdm2. Shown is a representative of 3 independent Western blot experiments. (B) BC3.14 cells were transfected with p53-TA-luc, a commercially available p53 reporter construct. Transfected cells were then divided and incubated with doxycycline (2 μg/mL for 48 hours), Groα (100 nM), or without additives as shown. Protein lysates were harvested, and equal amounts were assayed. Cells were divided after transfection, so no further control for transfection efficiency was performed. As expected, vGPCR expression has no effect on pTA-luc the control plasmid (inset). Shown is the average of 3 independent experiments. Error bars indicate SD.