KSHV vGPCR causes increase in p21 and p27. (A) BC3.14 cells were exposed to 2 μg/mL doxycycline for length of time shown in hours. Western blot shows vGPCR-dependent increases in p21 and p27 over 96 hours. Shown is 1 of 3 independent experiments. (B) Quantitative RT-PCR shows vGPCR-dependent transcriptional regulation of p21 and to a lesser extent, p27. Groα (100 nm), a vGPCR agonist, is included to assure specificity of observed effects. Average of 2 independent experiments each done in duplicate is shown. Error bars indicate SD. ^*Significant increase over baseline, P ≤ .05.