Lack of IL-7Rα does not affect the engraftment of LCs but does affect engraftment of epidermal αβ T cells. (A) IL-7Rα KO donor cells gave rise to epidermal LCs (major histocompatibility complex class II [MHC II]⁺ and CD45.2⁺) seen among host-derived LCs (MHC II single positive). Green is MHC II; red, CD45.2 (donor marker). Original magnification, × 20 for all images. Images were visualized under a Nikon TE300 microscope equipped with a 20 ×/0.75 objective lens (Nikon, Tokyo, Japan). A Coolsnap Pro digital camera was used to capture images (Roper Scientific, Duluth, GA), and IPlab software (Scanalytics, Fairfax, VA) was used to process them. Adobe Photoshop 7.0 software (Adobe, San Jose, CA) was used for subsequent image processing. (B-D) There was no significant difference in the chimerism of epidermal LCs when WT- and IL-7Rα KO-reconstituted mice are compared at both 4 and 8 weeks after reconstitution (B). There was little to no reconstitution of γδ T cells (C). (D) The major source for engrafted epidermal αβ T cells was of donor origin when WT donor cells were used and of host origin when IL-7Rα KO donor cells were used (n = 4 each).