Transcription factors NF-κB and NFAT bind to the BLYS promoter. (A) BLYS gene promoter diagram (GenBank accession no. AY129225) showing the putative binding sites for NF-κB (dots) and NFAT (black). (B) EMSA analysis of NF-κB binding to the BLYS promoter. Nuclear extracts from NHL-B (MS) cells were incubated with BLYS–NF-κB binding site oligonucleotides. BLYS-NF-κB cold probe and antibodies to p50, p52, p65, c-rel, or rel-B were added to the binding reaction mixtures. Arrows indicate the DNA-protein and supershifted complex. (C) EMSA analysis of NFAT binding to BLYS promoter. Nuclear extracts from NHL-B (LBCL-MS) cells were incubated with oligonucleotides for the 2 different BLYS-NFAT binding sites. BLYS-NFAT cold probes and antibodies to NFATc1, NFATc2, or NFATc3 were added to the binding reaction mixtures. Arrows indicate the DNA-protein and supershifted complexes.