BLT1 surface expression is increased on EBV-specific CD8⁺ T cells during acute EBV infection. (A) Scatter and lymphocyte gates showing increased numbers of circulating activated blasts (top left panel) and increased surface BLT1 expression on tetramer⁺ EBV-specific CD8⁺ T cells (bottom left panel) during acute EBV infection in R1 (FSC^lo), R2 (FSC^hi), and total (R1+R2) lymphocyte gates (right panel) in a representative sample. (B) BLT1 surface expression is significantly enriched on total and FSC^lo small lymphocyte-gated EBV-specific tetramer-positive CD8⁺ T cells (n = 4). (C) BLT1 surface expression is increased on tetramer⁺ EBV-specific CD8⁺ T cells during acute symptomatic EBV infection compared with the chronic phase of infection in healthy asymptomatic donors (P < .001); more Tet⁺ CD8⁺ BLT1⁺ T cells re-express CCR7 in chronic compared with acute infection. (D) Tetramer⁺ EBV-specific CD8⁺ T cells were gated on to compare surface chemoattractant receptor expression profile of EBV-specific HLA-B8–restricted QAK tetramer⁺ CD8⁺ T cells during acute infection (top panel), and EBV-specific HLA-B8–restricted RAK tetramer⁺ CD8⁺ T cells during the latent or chronic phase of infection (bottom panel). These data are representative of at least 3 donors.