BLT1 expression is significantly enriched on circulating FSC^hi large CD4⁺ and CD8⁺ lymphocytes. Representative data showing BLT1 expression as percent of quadrant on different T-cell types. (A) Cells were simultaneously gated on total lymphocyte and CD3 gates to characterize CD4⁺, CD8⁺, and γδ cells; and total lymphocyte and CD4 gates to characterize NKT cells. (B) Left panel: contour density plot of PBMC defining small FSC^lo, large FSC^hi, and total lymphocyte gates. Right panel: FACS plot demonstrating enrichment of surface BLT1 on FSC^hi large lymphocyte gated CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells. (C) BLT1 expression is significantly enriched on FSC^hi CD4⁺ and CD8⁺ T cells compared with total CD4⁺ and CD8⁺ T cells in freshly isolated PBMCs (n = 15). (D) FSC^hi CD4⁺ and CD8⁺ T cells express increased amounts of BLT1 mRNA by quantitative real-time PCR. These data are representative of 3 separate experiments.