Figure 2.
![Figure 2. DCs gain T-cell stimulatory capacity after exposure to PR3. Mature DCs obtained by TNF-α, PR3, and TNF-α+ PR3 exposure (from day 4 to day 8) were pulsed with PR3 on day 3 of their culture, and were plated at day 6 with autologous CD4+ T cells for 24 hours. Subsequently, the cells were subjected to the IFN-γ/IL-4 capture assay. The cells are labeled as described in “Patients, materials, and methods,” and then analyzed by flow cytometry. In order to confirm that the effect observed for PR3 is specific for this serine protease, similar experiments using HLE were also carried out. Data are plotted as the mean percentage of IFN-γ–producing cells ± SEM (healthy controls [HC], n = 5; WG, n = 10; CD, n = 6). *P < .05.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-05-1875/4/zh80110696090002.jpeg?Expires=1722703403&Signature=uhtw1mKQZBVFilcjgV~5M9YIIcs28e52M6A0ysNkJx7TLW5RKIeHcKXodpDCEkAT1-kYY-Wq-F9tIfwHL~KXqhelzCG-GCktwEPVFZe7igfxp5MrIBORh8WPg6uV1pczTnpAk1Rbm1fGf4RcQQ8GbHzF2QgVZ9ghGeyWAc6yv7GQ1XEKL7KKtgwhkmuF0Av2slNLqWi9hPzDBdOWAit5X9pRJ1TC8IdZPElSmvFpYuRRmmWrHOIhuBElMlUvbIyrXrIT193sfZSxyoGEmgfbISOqBXPj7KMUl3vMhkhC4x3cnXhOJao9bAE8qbJ3DPYx5ylNF9~VfG-lJCsSfrBsrQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DCs gain T-cell stimulatory capacity after exposure to PR3. Mature DCs obtained by TNF-α, PR3, and TNF-α+ PR3 exposure (from day 4 to day 8) were pulsed with PR3 on day 3 of their culture, and were plated at day 6 with autologous CD4+ T cells for 24 hours. Subsequently, the cells were subjected to the IFN-γ/IL-4 capture assay. The cells are labeled as described in “Patients, materials, and methods,” and then analyzed by flow cytometry. In order to confirm that the effect observed for PR3 is specific for this serine protease, similar experiments using HLE were also carried out. Data are plotted as the mean percentage of IFN-γ–producing cells ± SEM (healthy controls [HC], n = 5; WG, n = 10; CD, n = 6). *P < .05.
