Dual gene expression in human T cells. PBLs from 2 donors were nucleofected with the SB dual gene plasmid pT2/EGmCN (5 μg) ± pUbSB10 (10, 5, 2.5, and 0 μg). The cells were activated by anti-CD3/28 beads on day 2 and assayed for eGFP and NGFR expression on days 7 (data not shown) and 21. Similar data were also obtained from another donor.