NIMA exposure reduces T-cell proliferative and cytokine responses to NIMAs. CD4⁺ T cells isolated from spleens of NIMA-exposed and control B6 mice were cultured with B6D2F1 DCs for 72 hours. Supernatants were collected for the measurement of IFN-_γ levels, and the cells were pulsed with ³[H]thymidine to determine cell proliferation. Proliferation (A) and IFN-_γ levels (B) are shown as mean ± SD. (C-D) A total of 5 × 10⁵ CD4⁺ T cells isolated from spleens from NIMA-exposed and control mice were adoptively transferred into lethally irradiated B6D2F1 mice (C) or third-party B6C3F1 mice (D). Five days later, donor CD4⁺ T-cell expansion as determined by H-2^d- CD4⁺ in MLNs was determined. ^*P < .05.