Amino acid sequence alignment of the ATP-binding site region of human JAK2 PTK domain with the other members of the JAK family TYK2, JAK3, and JAK1 and the kinase domain of FAK, IRK, ZAP-70, FGFR2, and LCK. The secondary structure of JAK2 is illustrated directly above the sequence alignment. Arrows delineate β-strands, and cylinders delineate α-helices. Dark gray boxes indicate conserved residues; light gray boxes, conservatively substituted residues. Residues highlighted in blue are located in the adenine-binding region; in green, in the sugar pocket; and in pink, in the phosphate-binding region. Residues accessible to solvent are colored in brown and buried residues are colored in purple. IC50s of CMP6 for each kinase are indicated on the right.
