American Society of Hematology

Figure 7.

$Figure 7. Expression level of Tmtsp/Venus correlates to hematopoietic activity. (A) Expression of Venus in hematopoietic stem and progenitor cells. (B) Expression of Venus in hematopoietic progenitors. Bone marrow–derived IL-7R–Lin– Sca-1–c-Kit+ cells were subdivided into FcγRlowCD34+ (CMP), FcγRhighCD34+ (GMP), and FcγRlowCD34– (MEP) fractions (top left), and the expression of Venus is shown in the histograms (top right). Venus signal in IL-7R+Lin–c-KitlowSca-1low cells (CLP) (bottom panel). (C) Colony-forming cells were highly enriched in the Lin–Venushigh fraction. Numbers of HPP-CFCs and LPP-CFCs are presented as the mean value ± SD of triplicate cultures. Numbers of HPP- and LPP-CFCs in the Lin–Venushigh fraction were significantly different from the Lin–Venuslow fraction (Student t test, ***P < .001, *P < .05).$

Expression level of Tmtsp/Venus correlates to hematopoietic activity. (A) Expression of Venus in hematopoietic stem and progenitor cells. (B) Expression of Venus in hematopoietic progenitors. Bone marrow–derived IL-7R^–Lin^– Sca-1^–c-Kit⁺ cells were subdivided into FcγR^lowCD34⁺ (CMP), FcγR^highCD34⁺ (GMP), and FcγR^lowCD34^– (MEP) fractions (top left), and the expression of Venus is shown in the histograms (top right). Venus signal in IL-7R⁺Lin^–c-Kit^lowSca-1^low cells (CLP) (bottom panel). (C) Colony-forming cells were highly enriched in the Lin^–Venus^high fraction. Numbers of HPP-CFCs and LPP-CFCs are presented as the mean value ± SD of triplicate cultures. Numbers of HPP- and LPP-CFCs in the Lin^–Venus^high fraction were significantly different from the Lin^–Venus^low fraction (Student t test, ***P < .001, *P < .05).

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