American Society of Hematology

Figure 6.

$Figure 6. Expression of Tmtsp marks hematopoietic progenitors in the developing embryo. (A) Expression kinetics of Venus, Flk-1, and CD41 in EBs (days 3-6). (B-D) Hematopoietic colony-forming cells were highly enriched in CD41lowVenushigh fraction. Yolk sac cells from E8.5 (B) and E9.5 (C) TmtspVenus/+ embryos and AGM cells from E10.5 TmtspVenus/+ embryo (D) were divided into 4 or 6 populations according to the expression levels of CD41 and Venus. Sorted cells were cultured on OP-9 stromal cells in the presence of SCF, TPO, IL-3, and EPO for 3 days. Sorting gates (left) and number of hematopoietic colony-forming cells per 1000 plating cells (right) are indicated. The results are shown as the mean value ± SD of triplicate cultures.$

Expression of Tmtsp marks hematopoietic progenitors in the developing embryo. (A) Expression kinetics of Venus, Flk-1, and CD41 in EBs (days 3-6). (B-D) Hematopoietic colony-forming cells were highly enriched in CD41^lowVenus^high fraction. Yolk sac cells from E8.5 (B) and E9.5 (C) Tmtsp^Venus/+ embryos and AGM cells from E10.5 Tmtsp^Venus/+ embryo (D) were divided into 4 or 6 populations according to the expression levels of CD41 and Venus. Sorted cells were cultured on OP-9 stromal cells in the presence of SCF, TPO, IL-3, and EPO for 3 days. Sorting gates (left) and number of hematopoietic colony-forming cells per 1000 plating cells (right) are indicated. The results are shown as the mean value ± SD of triplicate cultures.

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