Figure 3.
![Figure 3. Effect of various compounds on GC-box binding. EMSA with [32P]-labeled GC-box oligonucleotide was performed as follows: nuclear proteins (6 μg) from parental cells and their antifolate-resistant subline before and after treatment with Bt2-cAMP, IBMX, or pCPT-cGMP (A), pCPT-cGMP, pCPT-cGMP plus okadaic acid, or okadaic acid alone (B), were first incubated with [32P]-labeled GC-oligonucleotide, resolved by electrophoresis on polyacrylamide gels, and analyzed by a Phosphorimager.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/2/10.1182_blood-2005-07-2743/4/zh80020689610003.jpeg?Expires=1722560321&Signature=QeYiIRicp5DXu2cEL8VsAyNyyN8PwfxwqxWU85oRpLoFrbMC5u6YuCa4m5lxvXvbEP07TA1nq8oT8tEGqI6cP28gp~vADw0sy-QYvVjmxgcN-JBD4NuOEGHNT-zvJ2Uk4zUSGoRJN9KJhHNX8cB~bJYWNeJapnyQKKJknBhZrhjDY8gwXV6C-w6jgn5gse~1vxI8NZU-5HlH7KmbRtfc-j3Bjp4gALZUpO0JLaiRluUylmmWYcW9kItZzn57dhOoID34EEMNIJLYvpnitdG~qsrT6kcsSELAxtRydUpMB8CuCpWr4yKdOtmHY~4NTxkMk~wSHXGLzgzJQZ-slDCKaQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of various compounds on GC-box binding. EMSA with [32P]-labeled GC-box oligonucleotide was performed as follows: nuclear proteins (6 μg) from parental cells and their antifolate-resistant subline before and after treatment with Bt2-cAMP, IBMX, or pCPT-cGMP (A), pCPT-cGMP, pCPT-cGMP plus okadaic acid, or okadaic acid alone (B), were first incubated with [32P]-labeled GC-oligonucleotide, resolved by electrophoresis on polyacrylamide gels, and analyzed by a Phosphorimager.
