Intracellular distribution of tissue factor in fibroblasts. Fibroblasts were fixed, permeabilized, and stained with anti–human TF and an organelle-specific antibody. Left column represents TF staining; middle column, organelle-specific staining; and the right column, the overlay image (colocalization) of TF and organelle-specific marker. Organelle-specific antibodies/stain used as follows: SYTOX Green for nucleus, anti-EEA1 and anti-rab5 for early endosomes, anti-LAMP1 for lysosomes, anticalreticulin for endoplasmic reticulum, and anti–golgin-97 for the Golgi. Note: In contrast to EEA1, whose localization is limited to early endosomes, rab5 is also known to present in the cytoplasmic side of the plasma membrane and clathrin-coated vesicles.