Micrographs of liver biopsy sample showing the entity and the distribution of iron. Staining technique: Perl Prussian blue. (A) The liver has normal architecture. Iron is heavily distributed in zone 1, whereas zone 2 appears less loaded. Original magnification, × 25. (B) Most hepatocytes were massively loaded by hemosiderin (Sciot grading 3) and by Kupffer cells to a minor extent (Sciot grading 2). Original magnification, × 200. Images were observed using an Axioplan 2 microscope (Zeiss, Göttingen, Germany) and Plan-Apochromat 4×/0.10 numeric aperture (NA) and 20×/0.40 NA objectives. Images were captured using a Nikon Coolpix 950 (Nikon, Melville, NY), and processed with ACDsee version 6.0 software (ACD Systems, Saanichton, BC, Canada).