Generation of T-cell clones expressing CD16/γ chimeric molecules. Five days after lentiviral transduction with the CD16/γ gene (moi = 10:1), the T-cell clones were stained with CD16-PE (3G8) and analyzed by flow cytometry. Percentages of CD16/γ-positive cells are indicated for each clone. Clones 4 and 31 are 2 cytolytic CD4⁺ HLADPB1*0401–specific T-cell clones, clone 31-DO8 is a CD8⁺ HLA-A*0201/CMV-pp65^N9V–specific T-cell clone, and clone 18-DO259 is a CD4⁺ cytolytic EBV-specific T-cell clone.