Figure 4.
![Figure 4. Contribution of individual cytokines to enhanced HSPC transduction by LVs. (A) Percentage of GFP-positive cells in suspension culture and colonies (CFC) of CD34+ cells transduced with 108 TU/mL LV-GFP without (–cyt) or with the indicated cytokine, added separately (IL-6, SCF, TPO, Flt3L) or all together (+cyt), and with or without (NT [not treated]) 1μM MG132. (B) Mean fluorescence intensity (MFI) of suspension culture cells shown in panel A. One experiment of 2 performed with similar results is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-10-4047/4/zh80110696420004.jpeg?Expires=1725304410&Signature=dSNn1RdaeIYS-MQC2r7x~qRFC-DWLndLLbZockpdCs~fLMZK4oy6FWNuh6tycPJrY1T-~NdFu28jNfCvZEae~Y-fa8abWT0UMuSoYf~xKOVYi9TYhwwkNZCQTEl8gIbQfT2iL0-I-ulEzRIaOmUVk8OM~yMPVMNgbtQuihNyzNWnwytFM-XOluJ9yo36WUsq-VXUrMxJmyha5dXBaNPJM1IOQ2GHhrgAUIAkk3H3Ca9aCiYYtmwdp4VxXoCF0nzB7dYhxtG6fIFlz~e1CppAataH6-Cgsb1ghbD1YrPd-NIZIKvpSD8VzUuf61yU-lWMesziO7DN9Tw8o0geB5mJ3Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Contribution of individual cytokines to enhanced HSPC transduction by LVs. (A) Percentage of GFP-positive cells in suspension culture and colonies (CFC) of CD34+ cells transduced with 108 TU/mL LV-GFP without (–cyt) or with the indicated cytokine, added separately (IL-6, SCF, TPO, Flt3L) or all together (+cyt), and with or without (NT [not treated]) 1μM MG132. (B) Mean fluorescence intensity (MFI) of suspension culture cells shown in panel A. One experiment of 2 performed with similar results is shown.
