Figure 6.
![Figure 6. Effect of the plasminogen active site mutant, [D(646)E]-Plg, on apoptosis. U937 cells were either untreated (diamonds) or exposed to 10 ng/mL TNFα (squares) (A) or 50 μg/mL CHX (circles) (B) in the absence or presence of increasing concentrations of either the plasminogen active site mutant, [D(646)E]-Plg (closed symbols), or wild-type plasminogen (open symbols) for 24 hours at 37°C. The cells were analyzed by FACS with annexin V–FITC and PI. Data are shown as mean ± SE, n = 2, for a representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-07-2872/4/zh80110696210006.jpeg?Expires=1724227785&Signature=5GhVcHpfz0h-Oci8IDhpSD59bWDOyA0nuc5jjYD9sT8QqIfOPKzO7~R8l3p65xhB5AthDRUZz2DJlYUZsQsEAhsCH9EeD0Jj3KH3bPD1MBENUJkOWwatebNjDHJgPH1nPgnAAwcOIz7e9m8g~lLSKAXFPJerGuHiVLQaagwEpAml6WWuI1xLTWSR2NOeQ1OH60gNeQchoXqZ0AaBlPAK4Bcn76xpny80BT0uSWc-ZfBir~SyfgWirgBWIji6mgqCm1foDKZ4A9r0zled1k23RrTTzCwu~jknlJRRuTwqW2ZHQKabL4MRIxheuyRK~xvg6ypapDvEERC~ySKDad3U5w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the plasminogen active site mutant, [D(646)E]-Plg, on apoptosis. U937 cells were either untreated (diamonds) or exposed to 10 ng/mL TNFα (squares) (A) or 50 μg/mL CHX (circles) (B) in the absence or presence of increasing concentrations of either the plasminogen active site mutant, [D(646)E]-Plg (closed symbols), or wild-type plasminogen (open symbols) for 24 hours at 37°C. The cells were analyzed by FACS with annexin V–FITC and PI. Data are shown as mean ± SE, n = 2, for a representative of 3 independent experiments.
