Figure 5.
![Figure 5. SHIP–/–HSCs express lower levels of CXCR4 and VCAM-1 molecules as assessed by flow cytometry. (A) A representative histogram of CXCR4 (left) and VCAM-1 (right) expression on live (DAPI-) KTLS cells (isotype control [□]), SHIP–/– [▪], and WT [▦]). (B) The plots on the left represent the mean fluorescence intensity (MFI) of KTLS cells for CXCR4, VCAM-1, and VLA-4, respectively, and the plots on the right show the percentage of KTLS cells positive for VCAM-1 and VLA-4, respectively (SHIP–/– [▪], WT [▴]). Significance was established using the unpaired student t test (Prism 4): **P < .001 and +P < .05 (mean ± SEM; n ≥ 3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-12-5021/4/zh80110696400005.jpeg?Expires=1722387971&Signature=ITtiJ8IIJQqazrhMmCIZhDTPOtWsQOnuyXklRN0hw0rEPQBenpHdmkue5nHAHhzfcat27XDY1NP5uX6ZQImbm~K77f-yPcOIsqKkTuyIwjOkeJ5Rso3ymWiyO20-e3pJYHivnkX~UWEoBUjij-mlT8xYnyvhNonxbvsxh3a08viqsmzyGdlmMM-6fuoEFiQFNKy1u5iAsE59D4YtVfk7FNG80cotQrM--1y4HvVEf4tnqmhU-tQV6gujAwIsmshOS5R6ORieMD7qBWO~6Da6G~Zc38HTW6q3P~IwZXDBU9Gphedb04AQwV0pBSgt8pi2Xo4GoA95SQCIXeiNP5~glA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SHIP–/–HSCs express lower levels of CXCR4 and VCAM-1 molecules as assessed by flow cytometry. (A) A representative histogram of CXCR4 (left) and VCAM-1 (right) expression on live (DAPI-) KTLS cells (isotype control [□]), SHIP–/– [▪], and WT [▦]). (B) The plots on the left represent the mean fluorescence intensity (MFI) of KTLS cells for CXCR4, VCAM-1, and VLA-4, respectively, and the plots on the right show the percentage of KTLS cells positive for VCAM-1 and VLA-4, respectively (SHIP–/– [▪], WT [▴]). Significance was established using the unpaired student t test (Prism 4): **P < .001 and +P < .05 (mean ± SEM; n ≥ 3).
