![Figure 2. SHIP–/– WBM and purified HSCs have compromised reconstituting activity. (A) CRU activity calculated based on the percentage of global repopulation in the PB by donor WBM cells (SHIP–/– [▪] and WT [▦]). (B) Percentage of global repopulation of PB by SHIP–/– (▪) and WT (▦) donor in a CRU assay. (C) Percentage of lymphoid and myeloid PB cells derived from SHIP–/– (▪) or WT (▦) WBM 16 weeks after transplantation in a CRU assay. (D) FACS plots show the level of PB reconstitution 16 weeks after KTLS transplantation in a representative DC assay mouse using sorted SHIP–/– and WT KTLS cells. (E) Percentage of global reconstitution 16 weeks after transplantation of sorted KTLS (SHIP–/– [▪] and WT [▦]) (n = 11 over 2 different experiments). (F) Proportion of lymphoid and myeloid PB cells derived from SHIP–/– (▪) or WT (▦) WBM 16 weeks after KTLS transplantation. (G) Percentage of global reconstitution 12 weeks after sorted KFLS transplantation (SHIP–/– [▪] and WT [▦]) (n = 7). Significance was established using the unpaired student t test (Prism 4): **P < .001; *P < .01; ++P < .005; and +P < .05 (mean ± SEM).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-12-5021/4/zh80110696400002.jpeg?Expires=1722388642&Signature=tcT1hkodfpTok9F69mrqg3Rrl920HfcfbJ5Js4tkEBID-gYnfWyp-kJSawCSVUincyJNNrxJ4GaR~ENyvpLy5JFYkJD9VumALu~LgoG-Mdo5bzJURuTvXDestfzImbDwOoy~36e9HOl-yev47ngga8MH1yco-~MNu0jRgB~PV5lJ5ixm6o0A8LIQTOe9hb9LSqmcPbwsvhljmLrIvWkfVkej2g~KvPnlxUYIJ9jND7uw8gxOIOIphDLfc-ZIWDnhvB93S0lzcGLOPZXKyNkAQVmstcIQJRVUiLwd6qjNvsnYYLCQ4f62pxdOsa-5dnrzZaGC0ATqUMqN1wntC9Qneg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SHIP–/– WBM and purified HSCs have compromised reconstituting activity. (A) CRU activity calculated based on the percentage of global repopulation in the PB by donor WBM cells (SHIP–/– [▪] and WT [▦]). (B) Percentage of global repopulation of PB by SHIP–/– (▪) and WT (▦) donor in a CRU assay. (C) Percentage of lymphoid and myeloid PB cells derived from SHIP–/– (▪) or WT (▦) WBM 16 weeks after transplantation in a CRU assay. (D) FACS plots show the level of PB reconstitution 16 weeks after KTLS transplantation in a representative DC assay mouse using sorted SHIP–/– and WT KTLS cells. (E) Percentage of global reconstitution 16 weeks after transplantation of sorted KTLS (SHIP–/– [▪] and WT [▦]) (n = 11 over 2 different experiments). (F) Proportion of lymphoid and myeloid PB cells derived from SHIP–/– (▪) or WT (▦) WBM 16 weeks after KTLS transplantation. (G) Percentage of global reconstitution 12 weeks after sorted KFLS transplantation (SHIP–/– [▪] and WT [▦]) (n = 7). Significance was established using the unpaired student t test (Prism 4): **P < .001; *P < .01; ++P < .005; and +P < .05 (mean ± SEM).
