Protein expression of survivin in ATL. Whole-cell lysates (50 μg protein) from patients with ATL were separated by 12.5% SDS-PAGE and then transferred to a PVDF membrane. The transferred proteins were reacted with antibody against survivin (A) as described in “Patients, materials, and methods.” *Concentrations of survivin protein and mRNA are expressed relative to the survivin protein and mRNA levels in control normal cells, which were assigned values of 1. (B) A comparison between the survivin mRNA level (y-axis) and the protein expression level (x-axis) in 17 patients with ATL. The survivin mRNA level correlated with the protein level (r = 0.516, P = .033).