Fig. 6.
Type I IFNs stimulate expression of antiviral ISGs in J2E cells.
J2E cells were seeded at 1 × 105 cells/mL, stimulated with type I IFNs (100 IU/mL), and RNA extracted at indicated time points. RNA (20 μg) was electrophoresed on formaldehyde gels, Northern blotted, and hybridized with (A) PKR or (B) 2′5′OAS. GAPDH loading control as indicated.
