PTX3 influences the membrane expression of CD86, MHC class I, and class II molecules by maturing DCs. The expression of CD86 (y-axis; A), MHC class I molecules (HLA-A, -B, -C; y-axis; B), and MHC class II molecules (HLA-DR; C) was assessed in the presence of PTX3 (50 μM), LPS (10 ng/mL), apoptotic cells, or a combination of these stimuli. The effect of CRP (50 μM) on CD86 expression (y-axis) in the presence or in the absence of apoptotic cells and LPS is also reported (D). ^*P < .01, significantly different from control. Results represent the mean ± SD of three independent experiments.