32D FLT3-ITD and 32D FLT3-ITD-N676K cells show a similar biologic phenotype. (A) Analysis of colony formation. 32D cells expressing FLT3-ITD and FLT3-ITD-N676K were seeded in methylcellulose medium and incubated for 7 days at 37°C. Thereafter, numbers of colonies were counted and plotted. (B) Proliferation was measured at various time points from 0 to 96 hours using an MTS-based assay.