PKC412 dose-response curves of 32D cells expressing FLT3-ITD and FLT3-ITD-N676K. Cells were treated with and without PKC412 at increasing doses for 48 hours. (A) Proliferation using an MTS-based assay was measured at 0 and at 48 hours of incubation, and the percentage of proliferating cells relative to the control (no inhibitor) was plotted. (B) 32D cells expressing FLT3-ITD and FLT3-ITD-N676K were treated with and without PKC412A for 48 hours before assessing apoptosis using cell cycle analysis. Depicted is the proportion of cells in sub-G1.