RQ-PCR for BCL11B in acute and lymphoma types. RQ-PCR was used to detect expression levels of BCL11B in acute and lymphoma types of ATLL. Four patients with acute-type ATLL showed high levels of BCL11B expression, although none of them showed any genomic change at RP11-1127D7. The horizontal bar indicates the mean expression level (16.32). Eleven patients with lymphoma-type ATLL with gains at RP11-1127D7 and 10 patients without any genomic change are also shown. None of these patients showed any significant level of expression. For comparison, BCL11B expression was examined in 1 CD4⁺ PBMC sample and 2 PBMC samples (Normal). The 4 acute-type patients showed significantly higher expression than did the 21 lymphoma-type patients. (Acute type versus lymphoma type with 14q32 gain [+]: Mann-Whitney U test, P = .002; acute type versus lymphoma gain [–]: Mann-Whitney U test, P = .005).