Figure 4.
Figure 4. Fluorescent microscopy analysis of the transduction efficiency of canine VWD BOECs. Canine VWD BOECs were subjected to a centrifugal transduction (900g, 30 minutes, 32°C at an MOI of 20) with Lenti-CMV-GFP (left) or empty vector (right), and 7 days after transduction, the presence of GFP was analyzed by using a fluorescence microscope. Cell nuclei were stained with DAPI.

Fluorescent microscopy analysis of the transduction efficiency of canine VWD BOECs. Canine VWD BOECs were subjected to a centrifugal transduction (900g, 30 minutes, 32°C at an MOI of 20) with Lenti-CMV-GFP (left) or empty vector (right), and 7 days after transduction, the presence of GFP was analyzed by using a fluorescence microscope. Cell nuclei were stained with DAPI.

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