Phenotype of normal and VWD canine BOECs and normal human BOECs by phase contrast and fluorescence microscopy. Morphology (A), immunostaining for ac-LDL uptake (B), and VWF (C) on normal canine BOECs; morphology (D), immunostaining for ac-LDL uptake (E), and VWF (F) on canine VWD BOECs. Immunostaining for ac-LDL (G), VWF (H), CD31 (I), and CD144 (J) on normal human BOECs. Pictures are representatives of immunostainings of BOECs isolated from 4 different VWD type 3 dogs, from 1 normal dog, and from 3 healthy human control subjects. Magnification in panels B, C, E, and F is 5 times the magnification in panels A and D. (K) VWF in conditioned medium from normal canine BOECs, canine VWD BOECs, and normal human BOECs, harvested before passing the subconfluent monolayer of cells, and in conditioned medium alone. Data are mean ± SEM (n = 3) with concentration of bovine VWF (0.035 μg/mL) present in the conditioned medium not included.