Quantification of provirus integration and hRAG1 transgene expression. (A) The hRAG1 copy number was quantified on the DNA extracted from thymus, spleen, lymph nodes, and bone marrow cells (n = 9) 6 months after transplantation. (B) Correlation between hRAG1 copy number and CD4⁺CD8⁺ (DP) number in the thymus of RAG1-TM animals 6 months after gene transfer. (C) T cells, B cells, and granulocytes were purified from the spleen before DNA extraction to quantify transgene copy number. Data are expressed as means ± SD. (D) hRAG-1 and mRAG-1 expression were determined on the RNA extracted from thymus, spleen, lymph nodes, and bone marrow cells (n = 9 for RAG1-TM and n = 4 for C57BL/10) 6 months after transplantation. hRAG-1 transcript expression was compared with endogenous acidic ribosomal phosphoprotein expression (PO) and expressed as a percentage of a reference sample (RAG-1 expression in the thymus of a wild-type mouse).