FCM light-scattering analysis and cytology of Mac-1⁺/Gr-1^int cells. (A) FSC histograms of Mac-1⁺/Gr-1^int (box) or Mac-1⁺/Gr-1^high (broken box) cells collected from FPs of groups of 10 BALB/c mice on day 3 after inoculation with RQ461 or RM461 infection (inoculum of 1 × 10⁶ PFUs). All histograms are from gated cell populations as indicated by boxed areas (i,ii,iii). (B) FSC histograms of MACS-enriched Mac-1⁺/Gr-1⁺ cells (boxed areas i and ii) from PB on day 3 or day 5 after inoculation with RQ461 (inoculum of 1 × 10⁶ PFUs). (C) FCM analysis of BM cells from uninfected mice analyzed by FCM for Mac-1 and Gr-1 after gating on FSC^low or FSC^high populations (boxed areas i and ii), indicating the percentage of total Mac-1⁺/Gr-1^int cells (× 600) in each population. (D-F) May-Grunwald-Giemsa-stained cells from FPs or PB of groups of 20 mice at 48 hours after inoculation (inoculum of 1 × 10⁶ PFUs), prepared as described for Figure 2. (D) FSC^low/Mac-1⁺/Gr-1^int cells from FPs. (E) FSC^high/Mac-1⁺/Gr-1^int cells from FPs. (F) Mac-1⁺/Gr-1^int cells from PB.