Glycolytic flux in LSK⁺ and LSK^– cells. (A) A schematic diagram of the glycolytic pathway. Enzymes identified in the iTRAQ experiment are shown in red type, along with their relative expression ratio as LSK^–/LSK⁺, showing that all enzymes identified in this experiment are found at higher levels in LSK⁺ cells. (B) LSK⁺ and LSK^– cells were cultured for 3, 6, and 18 hours. Lactate production was measured in the medium and after 6 and 18 hours and was significantly different between LSK⁺ and LSK^– cells (P < .05). Error bars show standard error of the mean (n = 3). (C) Cytospin slides of LSK⁺ or LSK^– hematopoietic cells were immunostained for GLUT1. Single deconvolved images of hematopoietic cells stained for GLUT1 (green) and the nucleus (DAPI) are shown. Bar shown is 10 μM. (D) Image intensity analysis of cells showed LSK⁺ cells were significantly different from those of LSK^– (P < .05) for GLUT1 expression.