American Society of Hematology

Figure 6.

$Figure 6. Apoptosis induction in HTLV-1–transformed cells treated with shRNA specific for HIAP-1. The HTLV-1–transformed T-cell lines (MT-2 and C91-PL) and HTLV-1–negative control cells (HuT-78) were infected with lentiviruses containing shRNAs specific for HIAP-1 (siHIAP), nonsense shRNAs (sinon), empty lentiviruses (vector), and were mock-infected. (A) The apoptosis rate was determined by PI staining of nuclear DNA. The cells in the sub-G1 fraction represent apoptotic cells. As an apoptosis control, uninfected cells were treated with 1μg/mL etoposide (etop.) for 24 hours.$

Apoptosis induction in HTLV-1–transformed cells treated with shRNA specific for HIAP-1. The HTLV-1–transformed T-cell lines (MT-2 and C91-PL) and HTLV-1–negative control cells (HuT-78) were infected with lentiviruses containing shRNAs specific for HIAP-1 (siHIAP), nonsense shRNAs (sinon), empty lentiviruses (vector), and were mock-infected. (A) The apoptosis rate was determined by PI staining of nuclear DNA. The cells in the sub-G₁ fraction represent apoptotic cells. As an apoptosis control, uninfected cells were treated with 1μg/mL etoposide (etop.) for 24 hours.

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