Figure 5.
Poor proliferation of siHIAP1-containing HTLV-1–transformed cells. The HTLV-1–transformed T-cell lines (MT-2 and C91-PL) and HTLV-1–negative control cells (HuT-78) were infected with lentiviruses expressing shRNAs specific for HIAP-1 (siHIAP), nonsense shRNAs (sinon), empty lentiviruses (vector), and were mock-infected. Two days after infection, the cell density was adjusted to 1.5 × 105 cells/mL and then counted at the indicated time points. The figure shows growth curves obtained for unselected cultures and for cultures treated with blasticidin S hydrochlorid (selected). Mock infected control cultures died in the presence of blasticidin S hydrochlorid within 4 to 6 days (not shown).
