Augmented blood flow in tumors by subcutaneous transplantation of VPCs and expansion of transplanted VPCs within the tumor

. (A) LDPI analyses of blood flow in 3 VPC transplantation groups. Bilateral generation of C6-derived tumor (black circles) and unilateral transplantation of VPCs (arrowheads) in nude mice is shown. Differentiated VPC transplantation augmented blood flow of tumor (red to white colors) compared with control, whereas undifferentiated and PDGF-BB–treated VPCs did not (blue to green colors). (B) Quantitative analyses demonstrated the significant enhancement of differentiated VPC transplantation/control tumor blood flow ratios in nude mice. Data shown (mean ± SEM) are representative of 3 experiments. *P < .05. (C) Percents of the number of sectioned tumor blocks containing LacZ⁺ cells. About 40% of tumor blocks contained LacZ⁺ cells in differentiated VPC transplantation groups, whereas about 10% of them did in other VPC transplantation groups. The number of tumor blocks containing LacZ⁺ cells in differentiated VPC transplantation groups was significantly increased. Data shown (mean ± SEM) are representative of 3 experiments. *P < .01; NS,P > .05.