Id2, Tal1, and GATA1 protein expression as evaluated by Western blot.
(A) Id2, Tal1, and GATA1 expression from day 0 to day 14, 21, or 26 in minibulk HPC erythroid cultures supplemented or not with Dex at optimal dosage (10−7 M) KL or KL + Dex, respectively. C indicates control cultures. β-Actin controls are also included. Shaded area indicates sample not available (ie, the culture had already reached terminal maturation and had therefore been terminated). (B) Tal1 (top panel) and Id2 (bottom panel) expression in minibulk HPC erythroid cultures supplemented with KL and different doses of Dex (from 10−6 to 10−9 M) from day 7 to complete maturation (day 21 for KL ± Dex 10−6 M or 10−9 M and day 26 for KL + Dex 10−7 or 10−8 M, respectively). β-Actin controls are also included. Shaded area indicates sample not available.
