Fig. 2.
F-cell analysis.
(A) Percentage of F cells in minibulk unilineage erythroid cultures supplemented or not supplemented with KL ± graded amounts of Dex (10−9 to 10−6 M). C indicates control cultures. Mean ± SEM values from 5 separate experiments. **P < .01 and ***P < .001 when compared with KL alone. The difference between KL and KL + Dex 10−9 M and between C and all Dex dosages is not significant. (B) F-cell evaluation by indirect immunofluorescence labeling (top; original magnification × 400) or by flow cytometry (middle and bottom; thin lines indicate negative controls, and thick lines, anti-HbF Ab–treated cells). Representative results are shown.
