Detection of intracellular basic fibroblast growth factor (bFGF) in myeloma cells.

(A) Intracellular bFGF in U-266 myeloma cells stained by intracellular sandwich labeling using a polyclonal rabbit antihuman bFGF antibody and a goat antirabbit FITC-labeled secondary antibody. (B) Intracellular bFGF in sorted marrow myeloma cells from a representative patient (UPN no. 10). UC indicates unstained control; IC, isotype control.