Fig. 5.
ICAM-1, VCAM-1, and E-selectin gene expression in ECs cocultured with fibroblasts was not altered in response to static and flow condition.
ECs cultured alone (EC/φ) or cocultured with fibroblasts (EC/FB) were maintained in the static condition or exposed to shear stress of 12 dyne/cm2 for 6 hours and their mRNA expression was determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data shown are for a representative experiment. Similar results were obtained in a duplicate experiment.
