Fig. 4.
Effect of membrane pore size on the expression of ICAM-1, VCAM-1, and E-selectin in ECs cocultured with SMCs.
ECs were cultured alone (EC/φ) or cocultured with identical ECs (EC/EC) or SMCs (EC/SMC), using membranes containing (A) 3-μm or (B) 8-μm pores. Cocultured ECs were maintained in a static condition or exposed to shear stress of 12 dyne/cm2 for 6 hours and their mRNA expression was determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of cDNA was performed in parallel samples using human GAPDH primers. Data represent duplicate experiments with similar results.
